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The DNA sequencing lab contains three Beckman Biomek 3000 robots, used for setting up and cleaning up sequencing reactions, and two automated
ABI 3130XL DNA sequencers. All samples are submitted in bar coded tubes to ensure accurate sample handling and tracking. Read lengths of
about 1000 bases are attained by using LongTrace software from Nucleics. Turnaround time is generally 24-36 hours and the results are posted
on our server where both the text and chromatogram files can be viewed and downloaded.
Fill out our Online
DNA Sequencing Order Form to submit your DNA sequencing, PCR, or Gel-only order.
Information for submitting samples
All samples, both template and primer, should be submitted in bar-coded
tubes which we provide to you at no charge. Please stop by or contact us to receive these tubes.
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Plasmids:
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We
recommend using Qiagen mini or maxi kits. Concentration should be
determined by A260. Please adjust concentration to between 50ng/ul and
150ng/ul. Submit 300ng per sequencing reaction. |
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PCR
Products:
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Gel purification may or may not be necessary depending on how clean the
product is. Either way, the sample will need to be cleaned using
a product such as Quiagen's QIAquick kit. The concentration should be
between 10ng/ul and 150ng/ul and we need 10-150ng per sequencing
reaction depending on the length of the PCR product. |
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Primers:
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Please
adjust concentration to 5uM. This corresponds to 25ng/ul or 5 picomoles/ul. Submit 5ul per sequencing
reaction. |
We provide the following
standard primers at no cost. Please check the sequence with those in your
sample to ensure they are the same. Some companies have made changes without
changing the name of the primer.
| T7: |
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5'-TAA-TAC-GAC-TCA-CTA-TAG-GG-3' |
| T7 Term: |
5'-CTA-GTT-ATT-GCT-CAG-CGG-3' |
| T3: |
5'-ATT-ACC-CCT-CAC-TAA-AG-3' |
| M13F: |
5'-TGT-AAA-ACG-ACG-GCC-AGT-3' |
| M13R: |
5'-CAG-GAA-ACA-GCT-ATG-AC-3' |
| PGEX3: |
5'-CCG-GGA-GCT-GCA-TGT-GTC-AGA-GG-3' |
| PGEX5: |
5'-GGG-CTG-GCA-AGC-CAC-GTT-TGG-TG-3' |
| SP6: |
5'-ATT-TAG-GTG-ACA-CTA-TAG-A-3' |
High-Volume Service
If you have a large number of samples, 48 or more, you can submit them in a 96 well plate aliquoted at the correct concentration of template, primer, and water in each well. Please contact us for details.
Charges
$12 per reaction for Bac, Cosmids, and genomic DNA.
$9 per reaction for plasmids and pcr products.
$5 per reaction for high-volume service.
$5 per failed reaction (no data generated).
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